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  Transmission Electron Microscope

Transmission Electron Microscope:

The Transmission Electron Microscope is a complicated and highly advanced microscope (figure is as shown below). The Electron gun comprises a tungsten filament that whenever heated produces a beam of electrons that is then focused on the specimen by the condenser.

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   Figure: Illustrated Light pathway Comparison of Light Microscope and Transmission Electron Microscopy

Electrons cannot pass via the glass lens; therefore dough-nut-shaped electromagnets termed as magnetic lenses are employed to focus the beam. The electrons will be deflected by collisions with air molecules. Therefore, the column having the lenses and specimen is under high vacuum to acquire a clear image of the specimen on a fluorescent screen. The denser area in the specimen scatters more electrons and appears darker in the image since lesser electrons strike that region of the screen while electron transparent areas are brighter. The image captured at the screen can be made permanent on a photographic film.

Transmission electron microscopy contain high resolution and extremely helpful to observe various layers of specimens; though Transmission Electron Microscope has some drawbacks.

As electrons have limited penetrating power, only very thin part of the specimen (i.e., around100nm) can be studied efficiently. There is no 3-dimensional view. In addition specimens should be fixed, dehydrated and viewed under a high vacuum to prevent electron scattering. The procedure employed for specimen preparation, for viewing beneath Transmission Electron Microscope causes certain shrink- age and distortion.

 

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