Various Techniques of Immunofluorescence:
First utilized by Coon’s in the year 1942 for Pneumococcus in tissue sections.Direct method: or single layer method
In this technique antigen is treated once with antibody tagged with fluorochrome.
Illustration: Explanation of Rabies antigen in impression smear.1. Impression smear from hippocampus of infected dog is prepared and fixed.
2. This is treated with antirabies antibody tagged with Incubated and FITC.
3. It is washed to eliminate un-reacted antibody.
4. The preparation is inspected for fluorescence.
Likewise different other antigens can be explained.
1. To explain viruses and bacteria in the respiratory specimens.2. To explain Adenovirus, Chlamydia in the conjuntival smears.3. To explain GC and Mycoplasma in urethral smear.Indirect Technique or Antiglobulin Method or Double Layer Technique:
Principle:
To explain the antigen, first unlabelled particular antibody is employed. Subsequently it is treated with tagged antibody to gamma globulin. (Therefore when the first layer is rabbit antibody then the second layer is anti-rabbit gamma globulin- tagged).Procedure:
To explain an unknown antigen in fixed preparation. The test is completed in two steps:Step: 1
1. Unknown antigen is existed in a fixed preparation.
2. It is treated with particular non-fluorescent serum.
3. Incubated at 37ºC for 10 to 20 min in wet chamber.
4. Washed 3 to 4 successive baths for 5 min each in phosphate buffered saline. Antigen antibody complex is opposed to washings. The preparation is washed and then dried.Step 2:
1. To the washed dried preparation labeled anti-species anti-globulin is added and incubated at 37ºC for 15 to 30 minutes in wet chamber. It is then washed and dried as in step 1. Whenever antigen-antibody complex has been made in the first step, labeled antiglobulin will couple to particular antibody to antigen and a finely fluorescent complex will be prepared.
Latest technology based Biology Online Tutoring Assistance
Tutors, at the www.tutorsglobe.com, take pledge to provide full satisfaction and assurance in Immunology help via online tutoring. Students are getting 100% satisfaction by online tutors across the globe. Here you can get homework help for Immunology, project ideas and tutorials. We provide email based Immunology help. You can join us to ask queries 24x7 with live, experienced and qualified online tutors specialized in Immunology. Through Online Tutoring, you would be able to complete your homework or assignments at your home. Tutors at the TutorsGlobe are committed to provide the best quality online tutoring assistance for Microbiology Homework help and assignment help services. They use their experience, as they have solved thousands of the Biology assignments, which may help you to solve your complex issues of Immunology. TutorsGlobe assure for the best quality compliance to your homework. Compromise with quality is not in our dictionary. If we feel that we are not able to provide the homework help as per the deadline or given instruction by the student, we refund the money of the student without any delay.
tutorsglobe.com introduction of foreign gene assignment help-homework help by online recombinant dna technology tutors
tutorsglobe.com computing internal rate of return assignment help-homework help by online internal rate of return tutors
tutorsglobe.com decomposition of sulphur compounds assignment help-homework help by online sulphur cycle tutors
Nuclear Structure tutorial all along with the key concepts of nuclides with same Isotopes, nuclides with same Isobars, nuclides with same Isotones, uncertainty in momentum of electron, Excess mass and packing fraction, Nucleus, Nuclear Binding Energy and Separation Energy
Skeletal Biology Assignment Help - All That You Need Trusted Experts, Affordable Prices and High-Quality Work Promptly!
tutorsglobe.com conservation of energy assignment help-homework help by online natural resources tutors
www.tutorsglobe.com offers languages and reliability homework help, assignment help, case study, writing homework help, online tutoring assistance by computer science tutors.
Let’s turn your academic tasks woes into wins – Get reasonable Epic Poetry Assignment Help right away!
www.tutorsglobe.com offers other functional group homework help, other functional group assignment help, online tutoring assistance, organic chemistry solutions by online qualified tutor's help.
Microscopic Properties of Dielectrics tutorial all along with the key concepts of electric fields in cavities of a Dielectric, Clausius-Massotti Equation, Polarization in a Gas, Polarisability and Refractive Index and Dielectric Capacitor in our practical life
tutorsglobe.com zero based budgeting assignment help-homework help by online budget tutors
Transmission lines tutorial all along with the key concepts of wave equations of voltage and current, characteristic impedance of transmission line, Parallel wire and coaxial cable transmission line
tutorsglobe.com some early views of heredity assignment help-homework help by online heredity tutors
www.tutorsglobe.com assignment help tutorials, for various scientific method in operations research and role of computers in or. the scientific method in or study usually includes three phases.
Simple Experimental design-Analysis of Variance tutorial all along with the key concepts of Types of Experimental Design, Completely Randomized Design, Randomized Block Design, Simple Factorial Experiment, Analysis of Variance, Assumptions in ANOVA and Hypotheses in ANOVA
1949499
Questions Asked
3689
Tutors
1492308
Questions Answered
Start Excelling in your courses, Ask an Expert and get answers for your homework and assignments!!