Phase Contrast Microscope:
Phase contrast Microscope first explained in the year 1934 by Dutch Physicist Frits Zernike, is a contrast-improving optical method that can be employed to generate high-contrast images of transparent specimens.
The total detail of living cells is undetectable in bright field microscope as there is too little contrast among structures with identical transparency. Such organisms are noticed with a phase contrast microscope. The Phase Contrast Microscope is broadly employed for observing biological specimens. Phase Contrast Microscope is a kind of light microscope that improves the contrasts of colorless and transparent objects by influencing the optical path of light. In phase contrast microscope, the light passing via a transparent portion of the specimen travels slower.
The condenser of a phase contrast microscope contains an annular stop, an opaque disk with a thin transparent ring, that generates a hollow cone of light (figure is as shown).
Figure: The light pathway of Phase Contrast MicroscopyWhenever this cone of light passes via a cell, similar light rays are bent due to density variation and refractive index in the specimen. The deviated light is focused to build up an image of the object. Undeviated light rays hit a phase ring in the phase plate, located in the objective. Therefore in phase contrast microscope one set of light rays comes directly from the light source, and the other set of light comes from the reflected or diffracted from the specific structure of the specimen. Whenever two set of light rays are brought altogether they form an image of the specimen on the ocular lens, having regions that are relating light (i.e., phase) via the shades of gray to black. The interior structure of the specimen becomes more sharply stated in phase contrast microscopy.
Latest technology based Biology Online Tutoring Assistance
Tutors, at the www.tutorsglobe.com, take pledge to provide full satisfaction and assurance in General Microbiology help via online tutoring. Students are getting 100% satisfaction by online tutors across the globe. Here you can get homework help for General Microbiology, project ideas and tutorials. We provide email based General Microbiology help. You can join us to ask queries 24x7 with live, experienced and qualified online tutors specialized in General Microbiology. Through Online Tutoring, you would be able to complete your homework or assignments at your home. Tutors at the TutorsGlobe are committed to provide the best quality online tutoring assistance for Microbiology Homework help and assignment help services. They use their experience, as they have solved thousands of the Biology assignments, which may help you to solve your complex issues of General Microbiology. TutorsGlobe assure for the best quality compliance to your homework. Compromise with quality is not in our dictionary. If we feel that we are not able to provide the homework help as per the deadline or given instruction by the student, we refund the money of the student without any delay.
Technique of costing assists to represent the data in a specific format so that decision making becomes simple.
the theory and programs of arrays all along with the key concepts of arrays, character arrays, strings, subscripts, multiple-dimension arrays, programs, homework help and assignment help.
X-Ray Spectroscopy tutorial all along with the key concepts of Sources of X- rays, X-ray Emission Spectrometers, X-ray Detector, Non dispersive X-ray Spectrometers, X-Ray absorption, Application of X-ray Fluorescence Analysis
Where an intangible asset has a limited life, the approach taken for the depreciation (or amortisation as it is generally called with intangibles) is generally the same like that for property, plant and equipment (tangible non-current assets).
theory and lecture notes of static characteristics i all along with the key concepts of ideal inverter, logic voltages, drive capability, noise immunity, switching speed and bipolar transistor switch. tutorsglobe offers homework help, assignment help and tutor’s assistance on static characteristics i.
To make use of immersion water heater, one just fills a bucket of water and puts the immersion rod into the bucket either with an aid such as a clothes hanger or directly.
Tuned base oscillator tutorial all along with the key concepts of Circuit Action, Tuned Collector Oscillator, Positive Feedback, Hartley Oscillator, Colpitts Oscillator, Clapp Oscillator, Piezoelectric Effect, Q-factor
tutorsglobe.com characteristics of an isoquant assignment help-homework help by online isoquants tutors
theory and lecture notes of ttl inverter all along with the key concepts of circuit structure of ttl, logical operation of ttl, transfer characteristic of ttl and noise margins. tutorsglobe offers homework help, assignment help and tutor’s assistance on ttl inverter.
tutorsglobe.com structure of cell membrane assignment help-homework help by online cell membrane tutors
reactivity of alkaline earth metals tutorial all along with the key concepts of thermal stability of oxy salts, rock salt structure, wurtzite structure, beryllium hydride polymer
www.tutorsglobe.com offers Functional Budgets homework help, assignment help, case study, writing homework help, online tutoring assistance by accounting tutors.
www.tutorsglobe.com offers materials science homework help, materials science assignment help, online tutoring assistance, physical chemistry solutions by online qualified tutor's help.
The outermost covering of plants is termed as epidermal tissue system. It contains epidermis, stomata and epidermal outgrowths.
Comparative Morphology in Plant tutorial all along with the key concepts of Algae Morphology, Unicellular forms, Colonial Algae, Filamentous Forms, Heterotrichous Form, Thalloid Forms and Polysiphonoid Forms
1944399
Questions Asked
3689
Tutors
1453798
Questions Answered
Start Excelling in your courses, Ask an Expert and get answers for your homework and assignments!!