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  Laboratory diagnosis

Laboratory diagnosis:

1. A quick check for the presence of trypomastigotes is prepared by inspecting a wet blood film.

2. A thick and thin blood smears must be made and stained by Giemsa’s stain for T. cruzi.

3. A small quantity of blood is inoculated intraperitoneally into Guinea pigs. The animal blood is inspected for trypomastigotes after 7 days.

4. Xenodiagnosis: By permitting a laboratory-bred parasite free reduviid bug to feed on an individual suspected to be suffering from Chagas disease and 2 weeks later inspecting the intestinal contents for the existence of the parasite.

5. In late phases CSF may be observed for trypomastigotes by wet film inspection and staining.

6. Biopsy of an included lymph node or muscle (i.e., calf or deltoid) might reveal the existence of amastigote forms of T. cruzi.

Serologic diagnosis:

Complement fixation, indirect fluorescent antibody and indirect hemagglutination tests are routinely employed for the diagnosis of chronic Chagas disease.

Treatment and prevention:

Nifurtimox (i.e., Lampit) and benznidazole (i.e., radamil) decrease the severity of acute Chagas’ disease. Allopurinol is efficient in treating Chagas’ disease. Chagas disease is controlled via:

(1) Insecticides to abolish the reduviid vector.

(2) Construction of reduviid –proof dwellings

(3) and education.

 

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