Industrial production of penicillin:
Penicillin is generated on large scale in a commercially devised fermenter that gives optimum growth situation to Penicillium chrysogenum for maximum outcome.
Following are the steps included for the production of penicillin:
1) Inoculate 100 ml medium into 500 ml Erlenmeyer flask through spores of Penicillium chrysogenum strain and incubate at 25oC by keep them on the rotary shaker.
2) Subsequent to 4 days, transfer the content of container to the other container (4 liter capacity) having 2 liters of medium and incubate 2 days.
3) Transfer the substance to a stainless steel tank (i.e., 800 liter capacity) having 500 liters of medium. This tank is equipped in such a manner that it could give the optimum situation for fungal growth.
4) After 3 days, employ the contents for inoculation of around 1,80,000 liter medium kept in fermenter (i.e., 2,50,000 liter capacity). The later is equipped with automatic devices to optimum growth circumstances.
5) Filter the content of fermenter subsequent to 6 days incubation.Filtrate has penicillin. Extract the penicillin into amyl - or butyl acetate. From it transfer penicillin to aqueous solvent by extracting with phosphate buffer. From a butanol water mix crystallize the potassium penicillin.
Figure: Penicillin in production
Latest technology based Biology Online Tutoring Assistance
Tutors, at the www.tutorsglobe.com, take pledge to provide full satisfaction and assurance in Industrial Microbiology help via online tutoring. Students are getting 100% satisfaction by online tutors across the globe. Here you can get homework help for Industrial Microbiology, project ideas and tutorials. We provide email based Industrial Microbiology help. You can join us to ask queries 24x7 with live, experienced and qualified online tutors specialized in Industrial Microbiology. Through Online Tutoring, you would be able to complete your homework or assignments at your home. Tutors at the TutorsGlobe are committed to provide the best quality online tutoring assistance for Microbiology Homework help and assignment help services. They use their experience, as they have solved thousands of the Biology assignments, which may help you to solve your complex issues of Industrial Microbiology. TutorsGlobe assure for the best quality compliance to your homework. Compromise with quality is not in our dictionary. If we feel that we are not able to provide the homework help as per the deadline or given instruction by the student, we refund the money of the student without any delay.
tutorsglobe.com anterior urethra assignment help-homework help by online types of normal flora tutors
Get impeccable and authentic Human Rights Assignment Help service by highly talented tutors and score maximum marks!
The trend of genes / characters to be inherited together due to their position on similar chromosome is termed as linkage.
tutorsglobe.com management of non-hazardous wastes assignment help-homework help by online waste management tutors
concept of temperature tutorial all along with the key concepts of concept of temperature and heat, thermal equilibrium, zeroth law of temperature, scale of temperature, specification of fixed points, specification of interpolation
tutorsglobe.com cell membrane assignment help-homework help by online biochemistry tutors
According to the devices employed the television receivers can be categorized into three types - Valve type receivers, Solid state receivers, Hybrid type receivers
Polyurethanes, Cellulose-Polyacrylonitrile tutorial all along with the key concepts of Polyurethanes, Spandex, Cellulose, Polyacrylonitrile, Polymers an Application
Important Terms Used In Joint Product and By-Products - Split Off Point, Joint Costs - all costs acquired before or up to the split off point are known as joint costs or pre separation costs.
tutorsglobe.com shape of d-orbitals assignment help-homework help by online shapes of orbitals tutors
Code division multiple access is abbreviated as CDMA is a channel access method used by several radio communication technologies.
avail algebra assignment help service by qualified and skilled tutors to get authentic papers at fair prices and to boost up your grades.
tutorsglobe.com blood group typing assignment help-homework help by online tissue transplantation tutors
tutorsglobe.com formation of the cell wall assignment help-homework help by online cell wall tutors
Diagrams might be of different types, One-Dimensional Diagrams,Two-Dimensional Diagrams, Three-Dimensional Diagrams, Pictograms, Cartograms or Map Diagrams. Costing data are usually presented by one-dimensional or two-dimensional diagrams.
1949047
Questions Asked
3689
Tutors
1469166
Questions Answered
Start Excelling in your courses, Ask an Expert and get answers for your homework and assignments!!