Actinomycotic Granules:
A) Yellow or white or pink granules are generated by them. For isolation SDA medium with or without antibiotics is employed. The
B) plates are incubated at room temperature and 37°C.
C) Biochemical test are utilized mainly for Nocardia and Streptomyces species.
D) Animal pathogenicity is completed for Nocardia species for which swiss mice are employed.Cultures:
Samples must also be cultured at 25 to 30°C and 37°C, on Brain heart infusion agar or blood agar for Actinomycetes and on Sabouraud’s agar for Eumycotic agents. The fungi which cause eumycetoma are all septate moulds. They emerge in culture within 1-4 weeks. Serological precipitin tests are employed to distinguish between eumycetoma and actionomycetoma and also to recognize the specific organisms. However they are of little value for diagnosis in routine usage.
Treatment:
Eumycetoma does not respond to chemotherapy and radical surgery is generally essential. For cutaneous form treatment with potassium iodide is satisfactory. In disseminated disease intravenous amphotericin B is needed.Epidemiology and Control:
Organisms generating mycetoma take place in soil and on vegetation. Barefoot farm laborers are mainly exposed. Proper cleaning of wounds and wearing shoes are logical control measures.
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