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  Action of restriction enzyme

Action of restriction enzyme:

Fragments of DNA from dissimilar organisms or even from dissimilar species might be joined together at their sticky ends, thus generating recombinant DNA. This is made probable through the use of an enzyme termed as ligase. Therefore, ligase is employed to join the two DNA fragments.

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                                                                Diagram: Action of restriction enzyme

Restriction enzyme and ligase do not concerned regarding to the source of DNA. When the exact sequence of nucleotides is met with through the particular restriction endonuclease, it cuts it. Likewise when the correct sequences of nucleotides at their sticky ends in the two strands are met with, these are ligated (joined) through the enzyme ligase.

 

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