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  Mono phosphate cleavage

Mono (ortho) phosphate cleavage and Pyrophosphate cleavage:

ATP may go through either an orthophosphate or pyrophosphate cleavage throughout its use in biosynthetic pathways. In an ATP molecule, while the terminal phosphate is cleaved it is known as mono phosphate/ortho phosphate cleavage.

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Though, in many ATP utilizing reactions in place of one terminal phosphate two terminal phosphate groups are enzymatically hydrolyzed to provide a pyro phosphate molecule and a large amount of energy that is greater as compared to the mono phosphate/ortho phosphate cleavage.

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Pyrophosphate (PPi) is frequently the product of a reaction which requires a driving force. The spontaneous hydrolysis of it, catalyzed via Pyrophosphatase enzyme, drives the reaction to which PPi is a substrate. The DG that is free energy for this pyrophosphate cleavage is 10.0 Kcal/mol and so an extra thermodynamic push is provided to specific enzymatic reactions that need more energy as compared to that of a mono phosphate cleavage and make sure the completeness of specific biosynthetic reactions.

 

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