--%>
Assignment Help - homework help

Problem on MM equation

How to obtain relation between Vm and Km,given k(sec^-1) = Vmax/mg of enzyme x molecular weight x 1min/60 sec S* = 4.576(log K -10.753-logT+Ea/4.576T).

E

Expert

Verified

According to M.M Equation,
                                
                           V = Vmax x {S]/Km + {S]
                
Since Vmax = ksec^-1 {Et}

V = Ksec^-1{Et} x {S}/Km + {S}

Substituting the given value of Ksec^-1 in the above equation,we get
 
V = Vmax x mol.wt x 1min/60 sec x {S}/mg of enzyme / Km +{S]

V = Vmax x mol.wt x {S}/mg of enzyme/Km +{S}

Substituting the given value of {S}

V = Vmax x mol.wt x 4.576(log K -10.753-logT+Ea/4.576T)/mg of enzyme/ Km +  4.576(log K -10.753-logT+Ea/4.576T)

   Related Questions in Chemistry

  • Q : Dissolving Group IV Carbonate Explain

    Explain how dissolving the Group IV carbonate precipitate with 6M CH3COOH, followed by the addition of extra acetic acid.

    		•
  • Q : Neutralisation of phosphorous acids

    Provide solution of this question. To neutralise completely 20 mL of 0.1 M aqueous solution of phosphorous acid (H3 PO3) the volume of 0.1 M aqueous KOH solution required is: (a) 40 mL (b) 20 mL (c) 10 mL (d) 60 mL

    		•
  • Q : What is ortho effect? Orthosubstituted

    Orthosubstituted anilines are generally weaker bases than aniline irrespective of the electron releasing or electron withdrawing nature of the substituent. This is known as ortho effect and may probably be due to combined electronic and steric factors.The overall basic strength of ort

    		•
  • Q : What is solvent dielectric effect?

    Ionic dissociation depends on the dielectric constant of the solvent.The Arrhenius that ions are in aqueous solutions in equilibrium with parent molecular species allows many of the properties of ionic solutions to be understood. But difficulties began to

    		•
  • Q : Dipole attractions for london dispersion

    Illustrate how are dipole attractions London dispersion forces and hydrogen bonding similar?

    		•
  • Q : Forms a molecule to an organic molecule

    Briefly state what forms a molecule to an organic molecule?

    		•
  • Q : Molal concentration Select the right

    Select the right answer of the question. If one mole of any substance is present in of solvent, then: (a) It shows molar concentration (b) It shows molal concentration (c) It shows normality (d) It shows strength.

    		•
  • Q : Molarity based question Help me to

    Help me to solve this problem. 4.0 gm of NaOH are contained in one decilitre of solution. Its molarity would be: (a) 4 M (b)2 M (c)1 M (d)1.5 M

    		•
  • Q : Product of HCl Zn Illustrate  the

    Illustrate  the product of HCl Zn?

    		•
  • Q : Normality of solution containing

    Can someone please help me in getting through this problem. Determine the normality of a solution having 4.9 gm H3PO4 dissolved in 500 ml water: (a) 0.3  (b) 1.0  (c) 3.0   (d) 0.1

    		•