--%>
Assignment Help - homework help

Problem on MM equation

How to obtain relation between Vm and Km,given k(sec^-1) = Vmax/mg of enzyme x molecular weight x 1min/60 sec S* = 4.576(log K -10.753-logT+Ea/4.576T).

E

Expert

Verified

According to M.M Equation,
                                
                           V = Vmax x {S]/Km + {S]
                
Since Vmax = ksec^-1 {Et}

V = Ksec^-1{Et} x {S}/Km + {S}

Substituting the given value of Ksec^-1 in the above equation,we get
 
V = Vmax x mol.wt x 1min/60 sec x {S}/mg of enzyme / Km +{S]

V = Vmax x mol.wt x {S}/mg of enzyme/Km +{S}

Substituting the given value of {S}

V = Vmax x mol.wt x 4.576(log K -10.753-logT+Ea/4.576T)/mg of enzyme/ Km +  4.576(log K -10.753-logT+Ea/4.576T)

   Related Questions in Chemistry

  • Q : Haloalkene with the help of polarity of

    with the help of polarity of c-x bond show that aryl halides are less reactive than alkyl halides

    		•
  • Q : Law of vapour pressure Select the right

    Select the right answer of the question. "The relative lowering of the vapour pressure is equal to the mole fraction of the solute." This law is called: (a) Henry's law (b) Raoult's law (c) Ostwald's law (d) Arrhenius's law

    		•
  • Q : Concentration of urea Help me to go

    Help me to go through this problem. 6.02x 1020 molecules of urea are present in 100 ml of its solution. The concentration of urea solution is: (a) 0.02 M (b) 0.01 M (c) 0.001 M (d) 0.1 M (Avogadro constant, N4= 6.02x 1023mol -1)<

    		•
  • Q : Nuclear Magnetic Resonance The nuclear

    The nuclear states produced by a magnetic field are studied in nuclear magnetic resonance spectroscopy.The frequency of the radiation that corresponds to the nuclear magnetic energy level spacings and the weakness of the radiation absorption that must be e

    		•
  • Q : What are methods of phenol preparation

    Phenol was initially obtained by fractional distillation of coal

    		•
  • Q : How molecule-molecule collisions takes

    An extension of the kinetic molecular theory of gases recognizes that molecules have an appreciable size and deals with molecule-molecule collisions. We begin studies of elementary reactions by investigating the collisions b

    		•
  • Q : Molar mass what is the equation for

    what is the equation for calculating molar mass of non volatile solute

    		•
  • Q : Polymers comparison of biodegradable

    comparison of biodegradable and non-biodegradable polymers

    		•
  • Q : Haloalkane how haloalkane can be

    how haloalkane can be prepared by refluxing alcohol with hydrohalic acids

    		•
  • Q : Molarity Give me answer of this

    Give me answer of this question. If 20ml of 0.4N, NaoH solution completely neutralises 40ml of a dibasic acid. The molarity of the acid solution is:(a) 0.1M (b) 0.2M (c)0.3M (d)0.4M

    		•