--%>

Problem on MM equation

How to obtain relation between Vm and Km,given k(sec^-1) = Vmax/mg of enzyme x molecular weight x 1min/60 sec S* = 4.576(log K -10.753-logT+Ea/4.576T).

E

Expert

Verified

According to M.M Equation,
                                
                           V = Vmax x {S]/Km + {S]
                
Since Vmax = ksec^-1 {Et}

V = Ksec^-1{Et} x {S}/Km + {S}

Substituting the given value of Ksec^-1 in the above equation,we get
 
V = Vmax x mol.wt x 1min/60 sec x {S}/mg of enzyme / Km +{S]

V = Vmax x mol.wt x {S}/mg of enzyme/Km +{S}

Substituting the given value of {S}

V = Vmax x mol.wt x 4.576(log K -10.753-logT+Ea/4.576T)/mg of enzyme/ Km +  4.576(log K -10.753-logT+Ea/4.576T)

   Related Questions in Chemistry

  • Q : Organic and inorganic substances living

    living beings are made up of organic and inorganic substances.according to their complexity of their molecules how can ach of these substances be classified?

  • Q : Explain Photoelectron Spectroscopy. The

    The energies of both the outer and inner orbitals of atoms and molecules can be determined by photoelectron spectroscopy.Energy changes of the outermost or highest energy electron of molecules were dealt with here in a different passion. The energies of ot

  • Q : Hydrocarbons list and identify

    list and identify differences between the major classes of hydrocarbons

  • Q : Mole fraction of urea Choose the right

    Choose the right answer from following. When 6gm urea dissolve in180gm H2O . The mole fraction of urea is : (a)10/ 10.1 (b)10.1/10 (c)10.1/ 0.1 (d) 0.1/ 10.1

  • Q : Molarity of HCl solution 20 ml of HCL

    20 ml of HCL solution needs 19.85 ml of 0.01M NaOH solution for complete neutralization. Morality of the HCL solution is:  (i) 0.0099 (ii) 0.099 (iii) 0.99 (iv) 9.9 Choose the right answer from above.

  • Q : Relative lowering of the vapour pressure

    Choose the right answer from following.The relative lowering of the vapour pressure is equal to the ratio between the number of: (a) Solute moleules and solvent molecules (b) Solute molecules and the total molecules in the solution (c) Solvent molecules and the tota

  • Q : Organic structure of cetearyl alcohol

    Show the organic structure of cetearyl alcohol and state what the organic family is? Briefly state it.

  • Q : Problem on molality Select the right

    Select the right answer of the question. Calculate the molality of 1 litre solution of 93% H2SO4 (weight/volume). The density of the solution is 1.84 g /ml : (a) 10.43 (b) 20.36 (c) 12.05 (d) 14.05

  • Q : Homework Silicon has three naturally

    Silicon has three naturally occurring isotopes. 28Si, mass = 27.976927; 29Si, mass = 28.976495; 30Si, mass = 29.973770 and 3.10% abundance. What is the abundance of 28Si?

  • Q : Problem on decinormal Select the right

    Select the right answer of the question. How much water is required to dilute 10 ml of 10 N hydrochloric acid to make it exactly decinormal (0.1 N): (a) 990 ml (b) 1000 ml (c) 1010 ml (d) 100 ml