--%>

Problem on MM equation

How to obtain relation between Vm and Km,given k(sec^-1) = Vmax/mg of enzyme x molecular weight x 1min/60 sec S* = 4.576(log K -10.753-logT+Ea/4.576T).

E

Expert

Verified

According to M.M Equation,
                                
                           V = Vmax x {S]/Km + {S]
                
Since Vmax = ksec^-1 {Et}

V = Ksec^-1{Et} x {S}/Km + {S}

Substituting the given value of Ksec^-1 in the above equation,we get
 
V = Vmax x mol.wt x 1min/60 sec x {S}/mg of enzyme / Km +{S]

V = Vmax x mol.wt x {S}/mg of enzyme/Km +{S}

Substituting the given value of {S}

V = Vmax x mol.wt x 4.576(log K -10.753-logT+Ea/4.576T)/mg of enzyme/ Km +  4.576(log K -10.753-logT+Ea/4.576T)

   Related Questions in Chemistry

  • Q : Wavelengths which the human eye can see

    Briefly state the wavelengths which the human eye can see?

  • Q : What is electrolytic dissociation? The

    The Debye Huckel theory shows how the potential energy of an ion in solution depends on the ionic strength of the solution.Except at infinite dilution, electrostatic interaction between ions alters the properties of the solution from those excepted from th

  • Q : Concentration of Barium chloride Give

    Give me answer of this question. If 5.0gm of BaCl2 is present in 106 gm solution, the concentration is: (a)1 ppm (b)5 ppm (c)50 ppm (d)1000 ppm

  • Q : Molecular Symmetry Types The number of

    The number of molecular orbitals and molecular motions of each symmetry type can be deduced. Let us continue to use the C2v point group and the H2O molecule to illustrate how the procedure develop

  • Q : Molar mass what is the equation for

    what is the equation for calculating molar mass of non volatile solute

  • Q : Procedure for separating the components

    Briefly describe the procedure for separating the components of the gun-powder?

  • Q : Ionization Potential Second ionization

    Second ionization potential of Li, Be and B is in the order (a)Li>Be>B (b)Li>B>Be (c)Be>Li>B (d)B>Be>Li

  • Q : Molality of a glucose solution What

    What will be the molality of a solution containing 18g of glucose (having mol. wt. = 180) dissolved in 500g of water: (i) 1m  (ii) 0.5m  (iii) 0.2m  (iv) 2m

  • Q : Relationship between free energy and

    The free energy of a gas depends on the pressure that confines the gas. The standard free energies of formation, like those allow predictions to be made of the possibility of a reaction at 25°C for each reagent at 

  • Q : Inorganic Chemistry Inorganic

    Inorganic Chemistry:In the year 1869, Russian Chemist Dmitry Mendeleyev forms the periodic table of the element. Since Newlands did before him in the year 1863, Mendeleyev categorizes the el