--%>

Problem on MM equation

How to obtain relation between Vm and Km,given k(sec^-1) = Vmax/mg of enzyme x molecular weight x 1min/60 sec S* = 4.576(log K -10.753-logT+Ea/4.576T).

E

Expert

Verified

According to M.M Equation,
                                
                           V = Vmax x {S]/Km + {S]
                
Since Vmax = ksec^-1 {Et}

V = Ksec^-1{Et} x {S}/Km + {S}

Substituting the given value of Ksec^-1 in the above equation,we get
 
V = Vmax x mol.wt x 1min/60 sec x {S}/mg of enzyme / Km +{S]

V = Vmax x mol.wt x {S}/mg of enzyme/Km +{S}

Substituting the given value of {S}

V = Vmax x mol.wt x 4.576(log K -10.753-logT+Ea/4.576T)/mg of enzyme/ Km +  4.576(log K -10.753-logT+Ea/4.576T)

   Related Questions in Chemistry

  • Q : Eutectic Formation In some two

    In some two component, solid liquid systems, a eutectic mixture forms.Consider, now a two component system at some fixed pressure, where the temperature range treated is such as to include formation of one or more solid phases. A simple behavior is shown b

  • Q : Mole fraction of solute The mole

    The mole fraction of the solute in 1 molal aqueous solution is: (a) 0.027 (b) 0.036 (c) 0.018 (d) 0.009What is the correct answer.

  • Q : Chem Silicon has three naturally

    Silicon has three naturally occurring isotopes. 28Si, mass = 27.976927; 29Si, mass = 28.976495; 30Si, mass = 29.973770 and 3.10% abundance. What is the abundance of 28Si?

  • Q : Problem on relative humidity Relative

    Relative humidity is the ratio of the partial pressure of water in air to the partial pressure of water in air saturated with water at the same temperature, stated as a percentage: Relative  =

    Q : Explain the process of coagulation of

    Presence of small concentrations of appropriate electrolyte is necessary to stabilize the colloidal solutions. However, if the electrolytes are present in higher concentration, then the ions of the electrolyte neutralize the charge on the colloidal particles may unite

  • Q : Determining concentration in ppm A 500

    A 500 gm tooth paste sample has 0.2g fluoride concentration. Determine the concentration of F in terms of ppm level: (a) 250 (b) 200 (c) 400 (d) 1000Answer: (c) F-ions in ppm = (0.2/500) x 106 = 400

  • Q : What are heterogenous catalysis? Give

    When the catalyst exists in a different phase than that of reactants, it is said to be heterogeneous catalyst, and the catalysis is called heterogeneous catalysis. For example, SO2 can be oxidized to SO3

  • Q : Molarity of the final mixture Can

    Can someone please help me in getting through this problem. Two solutions of a substance (that is, non electrolyte) are mixed in the given manner 480 ml of 1.5M first solution + 520 ml of 1.2M second solution. Determine the molarity of the final mixture

  • Q : Cons of eating organic foods Illustrate

    Illustrate the cons of eating organic foods?

  • Q : Calculating total vapour pressure

    Select the right answer of the question. The vapour pressure of two liquids P and Q are 80 and 600 torr, respectively. The total vapour pressure of solution obtained by mixing 3 mole of P and 2 mole of Q would be: (a) 140 torr (b) 20 torr (c) 68 torr (d) 72 torr