--%>
Assignment Help - homework help

Problem on MM equation

How to obtain relation between Vm and Km,given k(sec^-1) = Vmax/mg of enzyme x molecular weight x 1min/60 sec S* = 4.576(log K -10.753-logT+Ea/4.576T).

E

Expert

Verified

According to M.M Equation,
                                
                           V = Vmax x {S]/Km + {S]
                
Since Vmax = ksec^-1 {Et}

V = Ksec^-1{Et} x {S}/Km + {S}

Substituting the given value of Ksec^-1 in the above equation,we get
 
V = Vmax x mol.wt x 1min/60 sec x {S}/mg of enzyme / Km +{S]

V = Vmax x mol.wt x {S}/mg of enzyme/Km +{S}

Substituting the given value of {S}

V = Vmax x mol.wt x 4.576(log K -10.753-logT+Ea/4.576T)/mg of enzyme/ Km +  4.576(log K -10.753-logT+Ea/4.576T)

   Related Questions in Chemistry

  • Q : Microwave Adsorption The absorption of

    The absorption of microwave radiation increases the rotational energy of molecules and gives information about the moment of inertia of the molecules.Now we can begin the study of the spectroscopy that explores the different ways in which the energy of the

    		•
  • Q : Determining highest normality What is

    What is the correct answer. Which of the given solutions contains highest normality: (i) 8 gm of KOH/litre (ii) N phosphoric acid (iii) 6 gm of NaOH /100 ml (iv) 0.5M H2SO4

    		•
  • Q : Atmospheric pressure Give me answer of

    Give me answer of this question. The atmospheric pressure is sum of the: (a) Pressure of the biomolecules (b) Vapour pressure of atmospheric constituents (c) Vapour pressure of chemicals and vapour pressure of volatile (d) Pressure created on to atmospheric molecules

    		•
  • Q : Concentration of Sodium chloride

    Provide solution of this question. If 25 ml of 0.25 M NaCl solution is diluted with water to a volume of 500ml the new concentration of the solution is : (a) 0.167 M (b) 0.0125 M (c) 0.833 M (d) 0.0167 M

    		•
  • Q : Decision about dipole moment is present

    How can you decide if there is a dipole moment or not?

    		•
  • Q : Eutectic Formation In some two

    In some two component, solid liquid systems, a eutectic mixture forms.Consider, now a two component system at some fixed pressure, where the temperature range treated is such as to include formation of one or more solid phases. A simple behavior is shown b

    		•
  • Q : Vander Waals forces Wax is an example

    Wax is an example of: (a) Ionic crystal  (b) Covalent crystal  (c) Metallic crystal  (d) Molecular crystalAnswer: (d) Iodine crystals are molecular crystals, in which constituent particles are molecules having inter particle

    		•
  • Q : Problem related to molarity Provide

    Provide solution of this question. Increasing the temperature of an aqueous solution will cause: (a) Decrease in molality (b) Decrease in molarity (c) Decrease in mole fraction (d) Decrease in % w/w

    		•
  • Q : Strength of the Hydrochloric acid

    Provide solution of this question. 1.0 gm of pure calcium carbonate was found to need 50 ml of dilute HCL for complete reaction. The strength of the HCL solution is specified by : (a) 4 N (b) 2 N (c) 0.4 N (d) 0.2 N

    		•
  • Q : Number of mlecules in methane Can

    Can someone please help me in getting through this problem. The total number of molecules in 16 gm of methane will be: (i) 3.1 x 1023 (ii) 6.02 x 1023 (iii) 16/6.02 x 1023 (iv) 16/3.0 x 1023

    		•