--%>

Problem on MM equation

How to obtain relation between Vm and Km,given k(sec^-1) = Vmax/mg of enzyme x molecular weight x 1min/60 sec S* = 4.576(log K -10.753-logT+Ea/4.576T).

E

Expert

Verified

According to M.M Equation,
                                
                           V = Vmax x {S]/Km + {S]
                
Since Vmax = ksec^-1 {Et}

V = Ksec^-1{Et} x {S}/Km + {S}

Substituting the given value of Ksec^-1 in the above equation,we get
 
V = Vmax x mol.wt x 1min/60 sec x {S}/mg of enzyme / Km +{S]

V = Vmax x mol.wt x {S}/mg of enzyme/Km +{S}

Substituting the given value of {S}

V = Vmax x mol.wt x 4.576(log K -10.753-logT+Ea/4.576T)/mg of enzyme/ Km +  4.576(log K -10.753-logT+Ea/4.576T)

   Related Questions in Chemistry

  • Q : Explain the mechanism of Enzyme

    A mechanism for enzyme-catalyzed reactions that leads to the typical rate equation for these reactions can be described.A variety of rate equations are required to portray the rates of enzymes catalyzed reagents and physical conditions that are encountered

  • Q : Coordination compounds discuss

    discuss practical uses of coordination compounds

  • Q : What is solvent dielectric effect?

    Ionic dissociation depends on the dielectric constant of the solvent.The Arrhenius that ions are in aqueous solutions in equilibrium with parent molecular species allows many of the properties of ionic solutions to be understood. But difficulties began to

  • Q : HCl is an acid or a base Illustrate is

    Illustrate is HCl an acid or a base ?

  • Q : What do you mean by the term hydra What

    What do you mean by the term hydra? Briefly define it.

  • Q : What is depression in freezing point?

    Freezing point of a substance is the temperature at which solid and liquid phases of the substance coexist. It is defined as the temperature at which its solid and liquid phases have the same vapour pressure. The freezing point o

  • Q : BASIC CHARACTER OF AMINES IN GAS PHASE,

    IN GAS PHASE, BASICITIES OF THE AMINES IS JUST OPPOSITE TO BASICITY OF AMINES IN AQEUOUS PHASE .. EXPLAIN

  • Q : Problem based on molality of glucose

    Select the right answer of the question. If 18 gm of glucose (C6H12O6) is present in 1000 gm of an aqueous solution of glucose, it is said to be: (a)1 molal (b)1.1 molal (c)0.5 molal (d)0.1 molal

  • Q : Effect on vapour pressure of dissolving

    Give me answer of this question. When a substance is dissolved in a solvent the vapour pressure of the solvent is decreased. This results in: (a) An increase in the b.p. of the solution (b) A decrease in the b.p. of the solvent (c) The solution having a higher fr

  • Q : Explain alcohols and phenols in organic

    Alcohols and phenols are the compounds