PCR and its working
What is PCR and explain its working?
Expert
PCR, polymerase chain reaction, refers to the method which synthesizes several copies of the specific regions of DNA molecule called as target-regions.
First, DNA to be tested gets heated causing in the double helix to burst and the polynucleotide chains to get exposed. Then the small synthetic sequences of the DNA called as the primers and consisting of nucleotide sequences same as that of the sequences of extremities of the region to be studied are added. The primers that are paired along with the original DNA in extremities of the gene to be amplified. Enzymes termed as polymerases, which catalyze the DNA replication, and the nucleotide supply is added. The primers then are completed and chosen region is replicated. In presence of the more primers and more nucleotides millions of copies of that specific region are produced.
Explain, how chordates are reproduced?
Choosing a Scoring Matrix: All the scoring matrices will not perform similarly and may not be suitable in every situation. As noted above, Johnson and Overington compared results obtained with 12 difference scoring matrices, and Pearson also recently
Name the cells which generate the myelin sheath? Out of which substance is the myelin sheath made?
What are the fundamental constituents of cell membrane?
What happens whenever gastrointestinal exposure takes place?
Explain that how can an organism which once undergoes contact along with an antigen be immunized in opposition to potential infections by similar agent?
In order to remove barriers to communication an open door communication policy should be prepared and followed by managers at all levels. The superiors in the organization must create an atmosphere of confidence and trust in the organization so that the credibility gap may be narrowed down. Major
Where in eukaryotic cells does mRNA synthesis take place? To where do such molecules transfer?
As neurotransmitters are not consumed in synaptic process, name the mechanisms to decrease their concentrations in the synaptic cleft subsequent to they have been utilized?
Explain the circulatory system of birds?
18,76,764
1956389 Asked
3,689
Active Tutors
1411997
Questions Answered
Start Excelling in your courses, Ask an Expert and get answers for your homework and assignments!!