PCR and its working
What is PCR and explain its working?
Expert
PCR, polymerase chain reaction, refers to the method which synthesizes several copies of the specific regions of DNA molecule called as target-regions.
First, DNA to be tested gets heated causing in the double helix to burst and the polynucleotide chains to get exposed. Then the small synthetic sequences of the DNA called as the primers and consisting of nucleotide sequences same as that of the sequences of extremities of the region to be studied are added. The primers that are paired along with the original DNA in extremities of the gene to be amplified. Enzymes termed as polymerases, which catalyze the DNA replication, and the nucleotide supply is added. The primers then are completed and chosen region is replicated. In presence of the more primers and more nucleotides millions of copies of that specific region are produced.
Define the term CATH used in the Structural database of bioinformatics?
ADVANTAGE OF BIODIESEL: 1. Major advantage of biodiesel is its wide availability and renewable. 2. Dry land farming production of seeds like jatropha can increase agricultural o
Core competence: "A core competency is an area of specialized expertise that is the result of harmonizing complex streams of technology and work activity." Core competencies are the majority important value generat
Criticism of Taylor's Contribution Scientific Management has contributed a lot to the science of management but it was criticized on the following grounds: 1)
Function of Leadership The significance of leadership in management will be clear if we study the functions which are performed by a
How do you explain the term immunoglobulin?
How the circulatory systems participate within the functioning of endocrine system?
Describe osseous cavity and also state where the pituitary gland is positioned? Answer: The pituitary gland, or hypophysis, is positioned in the sella turcica of the
Explain difference between the chorion and amnion?
To which direction does growth of one side of a stem, branch or root persuade the structure to curve?
18,76,764
1956526 Asked
3,689
Active Tutors
1421347
Questions Answered
Start Excelling in your courses, Ask an Expert and get answers for your homework and assignments!!